Effect of DNA concentration on band intensity and resolution in agarose gel electrophoresis

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dc.contributor.authorArslan, Mevlut
dc.contributor.authorTezcan, Erdem
dc.contributor.authorCamcı, Haluk
dc.contributor.authorAvcı, Murat Kemal
dc.date.accessioned2025-02-09T14:33:17Z
dc.date.available2025-02-09T14:33:17Z
dc.date.issued2021
dc.departmentFakülteler, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü
dc.description.abstractObjective: Agarose gel electrophoresis (AGE) is a widely used method for separating, identifying, and purifying nucleic acids. It has been used intensively in molecular researches and biotechnological applications. In AGE, band resolution and band quality are important parameters for researchers. Until now, many factors such as comb thickness, gel concentration, voltage and buffers have been reported to influence resolution and band quality in AGE. However, effect of DNA concentration on band resolution and intensity in AGE is unclear. Therefore, aim of the study was to investigate the effect of DNA concentration on AGE results in detail. Materials and Methods: Different concentrations of DNA Marker and specific DNA fragments obtained by PCR were analyzed, but the loaded total DNA quantity was not changed. Furthermore, the effect of DNA concentration on AGE was also investigated by designed gel combs with different thicknesses and wideness. Findings: It was shown that DNA concentration did not affect gel resolution, and the effect was not changed by comb thickness and wideness. Also, 2-fold dilution did not affect band intensities while 8-fold dilution significantly affected band intensities of all tested DNA fragments. Conclusion: Concentrating samples, at least 8-fold, and using thin and narrow gel combs can be used for desired results in AGE.
dc.description.abstractObjective: Agarose gel electrophoresis (AGE) is a widely used method for separating, identifying, and purifying nucleic acids. It has been used intensively in molecular researches and biotechnological applications. In AGE, band resolution and band quality are important parameters for researchers. Until now, many factors such as comb thickness, gel concentration, voltage and buffers have been reported to influence resolution and band quality in AGE. However, effect of DNA concentration on band resolution and intensity in AGE is unclear. Therefore, aim of the study was to investigate the effect of DNA concentration on AGE results in detail. Material and Method: Different concentrations of DNA Marker and specific DNA fragments obtained by PCR were analyzed, but the loaded total DNA quantity was not changed. Furthermore, the effect of DNA concentra-tion on AGE was also investigated by designed gel combs with different thicknesses and wideness. Results: It was shown that DNA concentration did not affect gel resolution, and the effectwas not changed by comb thickness and wideness. Also, 2-fold dilution did not affect band intensities while 8-fold dilution signifi-cantly affected band intensities of all tested DNA fragments. Conclusion: Concentrating samples, at least 8-fold, and using thin and narrow gel combs can be used for de-sired results in AGE.
dc.identifier.doi10.52976/vansaglik.969547
dc.identifier.endpage333
dc.identifier.issn2667-5072
dc.identifier.issue3
dc.identifier.startpage326
dc.identifier.urihttps://hdl.handle.net/11501/1840
dc.identifier.urihttps://doi.org/10.52976/vansaglik.969547
dc.identifier.volume14
dc.institutionauthorTezcan, Erdem
dc.institutionauthorid0000-0001-7379-9931
dc.language.isoen
dc.publisherVan Yüzüncü Yıl University
dc.relation.ispartofVan Health Sciences Journal
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_DergiPark_20250209
dc.subjectAgarose Gel Electrophoresis
dc.subjectDNA Concentration
dc.subjectBand Quality
dc.subjectBand Intensity
dc.subjectGel Resolution
dc.subjectAgaroz Jel Elektroforezi
dc.subjectDNA Konsantrasyonu
dc.subjectBant Kalitesi
dc.subjectBant Işıması
dc.subjectJel Çözünürlüğü
dc.titleEffect of DNA concentration on band intensity and resolution in agarose gel electrophoresis
dc.title.alternativeAgaroz jel elektroforezinde DNA konsantrasyonunun bant yoğunluğu ve çözünürlüğü üzerine etkisi
dc.typeArticle

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