A universal self-eroding sacrificial bioink that enables bioprinting at room temperature

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dc.authoridAydin, Levent/0000-0002-2372-1711
dc.authorwosidAydin, Levent/IWD-9892-2023
dc.contributor.authorAydin, Levent
dc.contributor.authorKucuk, Serdar
dc.contributor.authorKenar, Halime
dc.date.accessioned2024-06-13T20:17:47Z
dc.date.available2024-06-13T20:17:47Z
dc.date.issued2020
dc.departmentİstanbul Gedik Üniversitesi
dc.description.abstractNatural polymer-based hydrogel bioinks are widely used in bioprinting due to their suitability for recapitulation of in vivo cellular activities. However, preservation of the target geometry in a cell-laden hydrogel is difficult to achieve. The aim of this study was to develop a universal sacrificial bioink that allows high cell viability and a better shape fidelity in the cell-laden construct. A polysaccharide-based universal sacrificial bioink was developed for microextrusion-based bioprinting and was optimized to erode in 48 hours in the cell culture medium without formation of any undesired by-products. The sacrificial hydrogel was prepared from alginate and agarose via a microwave oven assisted method and bioprinted at room temperature to generate microchannels in the cell-laden hydrogel or to support a tubular structure and its biocompatibility determined by live/dead assay. Bioprinting time was significantly reduced, down to a few minutes for a large-scale tissue model (1 minute 52 seconds for a 2 cm tubular structure), by means of a high bioprinting speed up to 25 mm/s. After 48 hours in the cell culture, the sacrificial bioink completely detached from the cell-laden construct without causing any changes in its printed shape. Cell viability in the cell-laden construct was observed to be more than 95% at the end of 3-day culture. This novel sacrificial bioink enables bioprinting at room temperature without affecting oxygen and nutrient penetration into the cell-laden hydrogel and allows retention of high cell viability and shape fidelity.
dc.description.sponsorshipKocaeli Universitesi [2016/006]
dc.description.sponsorshipThis study was funded by Kocaeli Universitesi (Contract number: 2016/006).
dc.identifier.doi10.1002/pat.4892
dc.identifier.endpage1647
dc.identifier.issn1042-7147
dc.identifier.issn1099-1581
dc.identifier.issue7
dc.identifier.scopus2-s2.0-85082820019
dc.identifier.scopusqualityQ2
dc.identifier.startpage1634
dc.identifier.urihttps://doi.org/10.1002/pat.4892
dc.identifier.urihttps://hdl.handle.net/11501/1062
dc.identifier.volume31
dc.identifier.wosWOS:000520747600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofPolymers for Advanced Technologies
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBioprinting
dc.subjectPrintability Score
dc.subjectSacrificial Hydrogel
dc.subjectShape Fidelity
dc.subjectUniversal Bioink
dc.subjectCell-Laden
dc.subjectTissue Constructs
dc.subjectCross-Linking
dc.subjectScaffolds
dc.subjectAlginate
dc.subjectGelatin
dc.subjectHydrogels
dc.subjectBone
dc.subjectBlends
dc.subjectGel
dc.titleA universal self-eroding sacrificial bioink that enables bioprinting at room temperature
dc.typeArticle

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