Recombinant production and characterization of a novel esterase from a hypersaline lake, Acıgöl, by metagenomic approach

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dc.contributor.authorTütüncü, Havva Esra
dc.contributor.authorBalcı, Nurgül
dc.contributor.authorTüter, Melek
dc.contributor.authorKaragüler, Nevin Gül
dc.date.accessioned2024-06-13T20:17:49Z
dc.date.available2024-06-13T20:17:49Z
dc.date.issued2019
dc.departmentFakülteler, Mimarlık ve Tasarım Fakültesi, Gastronomi ve Mutfak Sanatları Bölümü
dc.description.abstractThe aim of this study was to isolate a novel esterase from a hypersaline lake by sequence-based metagenomics. The metagenomic DNA was isolated from the enriched hypersaline lake sediment. Degenerate primers targeting the conserved regions of lipolytic enzymes of halophilic microorganisms were used for polymerase chain reaction (PCR) and a whole gene was identified by genome walking. The gene was composed of 783 bp, which corresponds to 260 amino acids with a molecular weight of 28.2 kDa. The deduced amino acid sequence best matched with the esterase from Halomonas gudaonensis with an identity of 91%. Recombinantly expressed enzyme exhibited maximum activity towards pNP-hexanoate with a k(cat) value of 12.30 s(-1). The optimum pH and temperature of the enzyme were found as 9 and 30 degrees C, respectively. The effects of NaCl, solvents, metal ions, detergents and enzyme inhibitors were also studied. In conclusion, a novel enzyme, named as hypersaline lake Acigol esterase (hAGEst), was identified by sequence-based metagenomics. The high expression level, the ability to maintain activity at cold temperatures and tolerance to DMSO and metal ions are the most outstanding properties of the hAGEst.
dc.description.sponsorshipIstanbul Technical University [39574]
dc.identifier.doi10.1007/s00792-019-01103-w
dc.identifier.endpage520
dc.identifier.issn1431-0651
dc.identifier.issn1433-4909
dc.identifier.issue5
dc.identifier.pmid31154531
dc.identifier.scopus2-s2.0-85066504082
dc.identifier.scopusqualityQ2
dc.identifier.startpage507
dc.identifier.urihttps://doi.org/10.1007/s00792-019-01103-w
dc.identifier.urihttps://hdl.handle.net/11501/1097
dc.identifier.volume23
dc.identifier.wosWOS:000480747900002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTütüncü, Havva Esra
dc.institutionauthorid0000-0003-3951-399X
dc.language.isoen
dc.publisherSpringer Japan KK
dc.relation.ispartofExtremophiles
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDegenerate Pcr
dc.subjectEsterase
dc.subjectGenome Walking
dc.subjectHalophiles
dc.subjectLake Acigol
dc.subjectMetagenomics
dc.titleRecombinant production and characterization of a novel esterase from a hypersaline lake, Acıgöl, by metagenomic approach
dc.title.alternativeRecombinant production and characterization of a novel esterase from a hypersaline lake, Acigol, by metagenomic approach
dc.typeArticle

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