A new turn off flurecnloset NIR probe for hypochlorous acid and its applications

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dc.contributor.authorIbrahimova, Ayten
dc.contributor.authorÖnem, Ayşe Nur
dc.contributor.authorAltun, Mehmet
dc.contributor.authorYeşil, Emin Ahmet
dc.contributor.authorBaysal, Aslı
dc.contributor.authorSaygın, Hasan
dc.contributor.authorSoyocak, Ahu
dc.contributor.authorÖzyürek, Mustafa
dc.date.accessioned2025-05-08T05:51:19Z
dc.date.available2025-05-08T05:51:19Z
dc.date.issued2025
dc.departmentMeslek Yüksekokulu, Gedik Meslek Yüksekokulu, Kimya Teknolojisi Programı
dc.description.abstractHypochlorous acid (HOCl) is a potent nonradical oxidant involved in various physiological processes, particularly within the human immune system. In this study, we introduce a novel, rapid, and highly efficient fluorometric method for the detection of HOCl. The method utilizes a near-infrared (NIR)-based fluorescent probe, NIR-QBH, which is characterized by its high sensitivity and chemical stability. NIR-QBH, containing olefinic C=C bonds, exhibits strong NIR emission at 660 nm (lambda ex = 618 nm). The detection mechanism relies on the oxidation of the C=C bond in the NIR-QBH structure by HOCl, resulting in the formation of non-fluorescent products. With a detection limit of 0.23 mu M, the probe demonstrates a fast response time of 4 min. Glutathione (GSH), an essential biothiol, was employed as a reference HOCl scavenger, and its HOCl scavenging activity was evaluated with an IC50 value of 8.97 mu M. Furthermore, the developed fluorometric assay was successfully applied for the detection of HOCl in fetal bovine serum (FBS) and aqueous solutions.
dc.description.sponsorshipIstanbul University-Cerrahpasa, Institute of Graduate Studies
dc.identifier.doi10.55730/1300-0527.3725
dc.identifier.issn1300-0527
dc.identifier.issue2
dc.identifier.urihttps://doi.org/10.55730/1300-0527.3725
dc.identifier.urihttps://hdl.handle.net/11501/2093
dc.identifier.volume49
dc.identifier.wosWOS:001476304300009
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.institutionauthorYeşil, Emin Ahmet
dc.institutionauthorid0000-0001-8559-1951
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council
dc.relation.ispartofTurkish Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHypochlorous Acid
dc.subjectNIR
dc.subjectFluorogenic Probe
dc.subjectGSH
dc.subjectCytotoxicity
dc.titleA new turn off flurecnloset NIR probe for hypochlorous acid and its applications
dc.typeArticle

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